Vol 20. N°1. 2019  |  Enero-Marzo de 2019


ARTÍCULOS ORIGINALES - NUTRICIÓN DE LA MADRE AL BEBÉ


ACTIVIDAD DE LA Α-AMILASA EN LECHE MATERNA EN LOS PRIMEROS 50 DÍAS POSTPARTO

Autores: GABRIELA MARÍA SILENZI USANDIVARAS, MARÍA EUGENIA PÉREZ, LILIANA CHIRENO


RESUMEN

Introducción: la α-amilasa es una enzima que tiene la capacidad de digerir uniones α (1,4). En la leche sus funciones son poco conocidas.
Objetivos: determinar la actividad de la α-amilasa en el suero lácteo humano.
Materiales y métodos: la medición se realizó con un kit comercial para determinación de amilasa sérica y se utilizaron muestras de leche humana.
Resultados: se encontró una actividad promedio de 2.000 U/l. La comparación de las medias de los valores de actividad desde el segundo al quinto día (1,372±0,796) con los correspondientes al lapso desde el sexto día al cuadragésimo séptimo (0,412±0,250) demostró que los valores eran signifcativamente diferentes (p<0,0025). Los coefcientes de variación de los dos grupos mencionados (1-5 días y 6-47 días) fueron 58 y 61% respectivamente. De manera adicional, la expresión de la amilasa mostró una gran variación entre madres.
Conclusiones: a partir de esta información y de los antecedentes bibliográfcos, la α-amilasa láctea tendría una función de defensa en el organismo del recién nacido lactante, y recién al iniciar la alimentación suplementada cumpliría el rol conocido. Aún así el signifcado biológico de su función en la leche es un problema abierto.

Palabras Clave: α-amilasa; enzimas; leche humana; calostro.

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ACTIVITY OF -AMYLASE IN HUMAN MILK IN THE FIRST 50 DAYS POSPARTUM

SUMMARY

Introduction: the α-amylase is an enzyme that has the ability to digest α (1,4) junctions. In milk, its functions are little known.
Objectives: the aim of this work was to determinate α amylase activity in human milk.
Materials and methods: the measurement was made with a commercial kit for serum amylase determination and samples of human milk were used.
Results: it was found an average activity of 2.000 U/l. The comparison of the means of activity values from the second to the ffth day (1.372±0.796) with those corresponding to the lapse from day 6 to 47 (0.412±0.250), showed that the values were signifcantly different (p<0,0025). The coeffcients of variation of the two groups mentioned (1-5 days and 6 to 47 days) were 58% and 61% respectively. Additionally, the expression of amylase showed great variation among mothers.
Conclusions: from this information and the bibliographic background, the milk α-amylase would have a defense function in the organism and only when starting the supplemented feeding would it fulfll the known role. Even so, the biological signifcance of its function in milk is an open problem.

Keywords: α-amylase; enzymes; human milk, colostrum.


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